Fig. 3
From: An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
The pNP system is superior to the pVALIUM20 system. a Knock down of E2F1 in the eye using the pVALIUM20 system or the pNP system with the same shRNA driven by ey-Gal4. Scale bars, 200 μm. b RNAi of Upf1 in the wing using MS1096-Gal4. Scale bars, 500 μm. c Targeting of aTub67C with the pVALIUM20 system or the pNP system driven by Nub-Gal4. Scale bars, 500 μm. d Knock down of egg in the germline controlled by nos-Gal4. Scale bars, 300 μm. e Fertility rates of control, pVALIUM20-egg and pNP-egg female flies using the nos-Gal4 driver (n = 10, mean ± s.d.). Data are evaluated with one-tailed Student’s t-test. f qRT-PCR analysis of the RNAi efficiency of E2F1, Upf1, aTub67C, and egg, respectively (n = 3, mean ± s.d.). Data are evaluated using one-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001)
