Fig. 5

Dose-dependent effect of rs116446171 variants on reporter transcription and cell proliferation. a Quantitative PCR analysis of EGFP transcripts in stably transduced cells with tandem repeats of the rs116446171 variant allele. The variant allele was inserted within the EXOC2 3′UTR region as a single copy or as two, four and eight repeats in either cis or trans orientation. Data are expressed as mean fold change of the endogenous controls, ±s.e.m., n = 9 replicates. ****P < 0.0001. b Proliferation assay of cells transduced with tandem repeats of the variant allele. Cells harboring eight tandem repeats proliferate significantly faster than cells harboring four tandem repeats, and cells harboring four tandem repeats proliferate significantly faster than cells harboring two or one repeat. Data are expressed as mean fold change of the cell line in the day seeded, ±s.e.m., n = 9 replicates. ****P < 0.0001. All P-values were calculated with unpaired t-test