Fig. 5
From: Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast
The torpedo nuclease Dhp1 is required for transcription termination of coding and ncRNA genes. a–c Normalized ChIP-seq signal of RNAPII subunits Rbp1 and Rbp3 in WT and Dhp1-depleted strains across the fba1 mRNA (a), the snR99 snoRNA (b), and the snu5 snRNA (c) genes grown in thiamine-treated minimal medium for 15 h. The dashed-line rectangles highlight delayed transcription termination in Dhp1-deficient cells. d–g Average ChIP-seq profile of Rbp1 and Rbp3 (d, e) or total RNAPII (Rbp1), Ser2-P, and Tyr1-P (f, g) in WT (solid lines) or Dhp1-depleted (dotted lines) cells across 4755 mRNA (d–f) and 24 monocistronic snoRNA (e, g) genes with a mapped p(A) site in thiamine-treated minimal medium
