Fig. 2

RYGB surgery effectively prevents the metastasis of pancreatic ACC. a Weight of pancreas, liver, and kidney. ^#P < 0.05 vs. WT. *P < 0.05 vs. Ngn3-Tsc1−/−(KO) sham. b Liver metastasis rate (left). c Liver gross and histological (H&E) morphology (right) in Ngn3-Tsc1−/−(KO) mice with sham or RYGB surgery. d Kidney metastasis rate (left). e Kidney gross and histological (H&E) morphology (right) in Ngn3-Tsc1−/−(KO) mice with sham or RYGB surgery. Values are mean ± SEM. n = 9, 5, 5 for WT sham, Ngn3-Tsc1−/−(KO) sham, Ngn3-Tsc1−/−(KO) RYGB, respectively, for male mice. n = 8, 5, 4 for WT sham, Ngn3-Tsc1−/−(KO) sham, Ngn3-Tsc1−/−(KO) RYGB, respectively, for female mice. Statistical differences were analyzed by one-way ANOVA followed by the Student’s t-test. *P < 0.05 vs. Ngn3-Tsc1−/−(KO) RYGB