Fig. 3

Functional analyses of BCL10-BCL10 interfaces in Jurkat T-cells. a, b BCL10 KO Jurkat T-cells lentivirally reconstituted with BCL10 wt, R42E, R36E or R42A constructs were stimulated P/I for 20 min and CBM complex formation was monitored by BCL10-IP (a) and Strep-tag II (ST) pull down (PD) (b). c, d BCL10 KO Jurkat T-cells reconstituted with BCL10 wt or BCL10-BCL10 interface mutants were stimulated with P/I for the times indicated. NF-κB activation was determined by IκBα WB and EMSA. Activation of the MALT1 protease was monitored by WB analyses of the substrate cleavage. Asterisks indicate non-specific cross-reactivity of the BCL10 antibody