Fig. 8
Treatment of orthotopic breast cancer and inhibitory effects on lung metastasis. a Tumor volume changes of the mice treated with PBS, light, cPt, MNPs, TPPNPs + L, or MNPs + L after one injection (n = 8). b Tumor weight of the mice treated with different formulations. c Kaplan–Meier survival curves of the mice bearing orthotopic 4T1 breast tumors treated with different formulations. d H&E staining of the tumor tissues from each group. Scale bar is 500 μM. e Representative images of the lungs excised from each group. The white circles denote the visually detected metastatic nodules in each lung tissue. f Histological examination of metastatic lesions in lung tissues from each group after H&E staining. Scale bar is 500 μM. g Photo images of the orthotopic tumors harvested from the mice treated with different formulations. Mice tails indicate no tumor founded. h The numbers of tumor nodules present in the lungs from each group. i Tumor coverage percentage in the lungs from each group. j PET/CT images of the mice treated with different formulations at the 14th day post injection. The white dash circles denote the orthotopic breast tumors, and the white solid circles denote the metastatic tumors. The laser density was 0.3 W cm–2 at 671 nm, and the irradiation time was 10 min. The data are expressed as means ± s.d., ***P < 0.001
