Fig. 1
From: S-nitrosylation of the zinc finger protein SRG1 regulates plant immunity
SRG1 expression is regulated by nitric oxide. a Transcript levels of SRG1 were determined following treatment with the nitric oxide (NO) donor, sodium nitroprusside (SNP), either alone or in combination with the NO scavenger 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO). b β-glucuronidase (GUS) activity upon SNP treatment of SRG1::GUS lines. GUS activity in the given SRG1::GUS line in response to SNP (300 μM) and SNP plus cPTIO (200 μM) was analyzed by a GUS activity assay. c Quantitative real time polymerase chain reaction (qRT-PCR) to quantify SRG1 gene expression post treatment with Pseudomonas syringae pv tomato DC3000 or Pst DC3000 expressing avrRpm1 in the presence or absence of cPTIO. d GUS activity assay of SRG1::GUS lines post treatment with either Pst DC3000 or Pst DC3000(avrRpm1) in the presence or absence of cPTIO. e Subcellular localization of SRG1-green fluorescent protein (GFP) and free GFP were analysed in Arabidopsis protoplasts using a confocal microscope. Scale bar, 5 μm. Error bars represent mean ± standard deviation (SD) (n = 3 independent experiments). Asterisks indicate a significant difference from mock (student t-test, ***P < 0.001, *P < 0.05)
