Fig. 2
From: S-nitrosylation of the zinc finger protein SRG1 regulates plant immunity
SRG1 is a positive regulator of plant immunity. a Morphological phenotype of CaMV35S::SRG1 overexpression lines. The indicated lines were soil-grown under short day conditions and photographed at 6-weeks-old. Scale bar, 2 cm. b, c The fresh weight (b) and mRNA level of SRG1 (c) in the stated Arabidopsis lines. Error bars represent ± SD from 3–6 biological replicates (t-test, ***P < 0.001, *P < 0.05, significantly different from Col-0). d, e Titre of Pst DC3000 (d) and Pst DC3000(avrRpm1) (e) in the indicated plant genotypes at 0 days post infiltration (dpi) and 3 dpi. Error bars represent ± SD from 6 biological replicates and student t-test shows significant difference from Col-0 (***P < 0.001, *P < 0.05). f Pathogenesis-related 1 (PR1) gene expression determined by quantitative real-time polymerase chain reaction (qRT-PCR) in the indicated plant lines. Error bars indicate ± SD (n = 3 and ***P < 0.001 by t-test compared with Col-0). g Total salicylic acid (SA) levels in the given plant genotypes. Error bars indicate ± SD (n = 3 and ***P < 0.001 by t- test compared with Col-0). h, i Accumulation of hydrogen peroxide and superoxide detected by either 3,3′-diaminobenzidine (DAB) (h) or nitro blue tetrazolium (NBT) (i) staining, respectively, in the given plant lines. Scale bar, 0.5 cm. j Cell death development was scored by trypan blue staining. Scale bar, 100 μm. k Ion leakage was recorded post Pst DC3000(avrRpm1) challenge at the indicated hours post inoculation (hpi). Error bars represent ± SD from 3 biological replicates. Asterisks indicate a significant difference compared with Col-0 (student t-test, ***P < 0.001, *P < 0.05)
