Fig. 3

LPS-activated NK cells inhibit clonal expansion of LCMV-specific P14 CD8 T-cells. P14 T-cells were transferred into the indicated recipients which were subsequently infected with LCMV. After one day, they received LPS or PBS as a control (ctrl). a Percentages P14 T-cells of splenocytes (left) and BrdU⁺ cells of splenic P14 T cells (right) at day 4 p.i. or day 4.5 p.i. respectively. For BrdU labeling, mice were injected with BrdU and sacrificed 20 min later. Data are pooled from 2 to 4 independent experiments with 1–3 mice per group (n = 5–9). b Percentages P14 T cells of splenocytes (left) and absolute numbers (#) of P14 T-cells in spleen (right) at day 5 p.i.. Data are pooled from seven independent experiments with 1–3 mice per group (n = 13–15). c Percentages P14 T cells of splenocytes in NK cell-depleted and non-depleted B6 at day 5 p.i.. d, e Percentages P14 T cells of splenocytes in IL-15^–/– and perforin^–/– mice at day 5 p.i.. Data are pooled from 2 to 3 independent experiments with 2–3 mice per group (n = 5–7). f CFSE-labeled P14 T cells were stimulated with splenic APCs from LCMV-infected B6 mice (day 4 p.i.) that had been injected with LPS (1 µg) or PBS at day 1 p.i.. After 3 days, cell division of P14 T cells was analyzed by dye dilution. Representative histograms are shown, numbers in histograms indicate mean values ± SEM; dots represent mean values of duplicates. Data are pooled from two independent experiments (n = 4) with 1–3 mice per group. *** p < 0.001; Mann–Whitney Test