Fig. 5
From: Bacterial coinfection restrains antiviral CD8 T-cell response via LPS-induced inhibitory NK cells
IL-15 blockade inhibits LPS-induced NK cell accumulation but not negative T-cell regulation. B6 mice were infected with LCMV and received LPS (a–c) or PBS (d–f) at day 1 p.i.. As indicated, groups of mice were additionally treated at day 1 and day 3 p.i. with mAb specific for IL-15/IL-15R complex. All mice were analyzed at day 8 after LCMV infection. a, d gp33- and np396-tetramer+ (tet) CD8+ T cells in percent of spleen cells in the indicated groups. b, e Percent NK cells (CD3–NK1.1+) of spleen cells. (c, f) Viral titers in spleen and liver. Data of individual mice from (a–c) two independent experiments with three mice per group (n = 6) and d–f one experiment with 2–3 mice per group (n = 2–3) are shown; horizontal bars represent the mean values. Dashed lines indicate detection limits. **p < 0.01, Mann–Whitney Test
