Fig. 2
From: Nanoscale metal-organic frameworks for mitochondria-targeted radiotherapy-radiodynamic therapy
In vitro subcellular localization of Hf-DBA and Hf-DBB-Ru. a Time-dependent enrichment of Hf-DBA or Hf-DBB-Ru in mitochondria. Mitochondria were isolated from nMOF treated cells and the nMOF amounts were quantified by ICP-MS, n = 3. b Time-dependent Pearson’s correlation coefficients. Co-localization of Hf-DBA-R or Hf-DBB-Ru with mitochondria was analyzed based on fluorescence images captured at 1, 2, 4, and 8 h incubation. N = 3. Representative mitochondria co-localization images of c–e Hf-DBA-R and f–h Hf-DBB-Ru by CLSM. Scale bar = 5 µm. Mitochondria were labeled with Rhodamine 123 in green (d, g). Two nMOFs emitted magenta signal (c, f). White areas merged from the magenta and green signals represent co-localization of Hf-DBA-R or Hf-DBB-Ru with mitochondria. Fluorescence topographic profiles (i, j) display fluorescence intensity curves of straight white lines marked in e and h, respectively. The dots and error bars represent individual data points and s.d. values, respectively. The CLSM images were obtained with two repetitions to afford similar results
