Fig. 3
From: Nanoscale metal-organic frameworks for mitochondria-targeted radiotherapy-radiodynamic therapy
In vitro anti-cancer efficacy via RT-RDT. a Cytotoxicity of Hf-DBA, H2DBB-Ru, and Hf-DBB-Ru upon X-ray irradiation at a dose of 2 Gy in MC38 cells, n = 6. b Clonogenic assay for evaluating radioenhancement upon X-ray irradiation on MC38 cells, n = 6. c γ-H2AX assays showing DSBs in MC38 cells treated with Hf-DBB-Ru or PBS and X-ray irradiation, confirming the radioenhancing effect. Scale bar = 20 µm. (+) and (−) refer to with and without irradiation, respectively. d 1O2 generation was detected using SOSG in live cells by CLSM. Scale bar = 10 µm. e Quantification of COX-2 by flow cytometry. The cells were incubated with PBS, Hf-DBA, H2DBB-Ru, and Hf-DBB-Ru and irradiated with X-rays at a dose of 2 Gy. f Annexin V/PI analysis of MC38 cells. Cells were incubated with PBS, Hf-DBA, H2DBB-Ru, and Hf-DBB-Ru with and without X-ray irradiation at a dose of 2 Gy. The quadrants from lower left to upper left (counter clockwise) represent healthy, early apoptotic, late apoptotic, and necrotic cells, respectively. The percentage of cells in each quadrant is shown on the graphs. The error bars represent s.d. values. The flow cytometry studies were obtained with two repetitions to afford similar results
