Fig. 7
From: Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters
OsHV-1 invades haemocytes of the susceptible SF11 oysters. OsHV-1 was detected in SF11 by in situ hybridization (a, c, e). Paraffin wax-embedded sections of oysters that were fixed 54 h after the beginning of the ‘natural’ experimental infection were hybridized with a specific probe labelled with digoxigenin and revealed using alkaline phosphatase-conjugated antibodies and NBT/BCIP (dark blue precipitate). Haemocytes were localized on consecutive tissue sections by immunostaining with an antibody specific to the SOD haemocytic protein (b, d, f). Immunostaining was revealed using alkaline phosphatase-conjugated secondary antibodies. Labelling of virus-infected cells was observed in areas enriched in haemocytes in different tissues (indicated by arrowheads), such as the connective tissue surrounding the digestive gland (a, b, scale bars 50 µM) or in the gills (c–f, scale bars 20 µM)
