Fig. 1
From: DHX9 helicase promotes R-loop formation in cells with impaired RNA splicing
Depletion of SFPQ impairs cell growth. a Western blot showing knockdown of SFPQ using different siRNAs. Expression of myc-SFPQ is resistant to knockdown by siSFPQutr. b Cell proliferation in U2OS cells transfected with different siRNAs targeting SFPQ and a non-specific sequence (siControl). Cell number was measured 48 h after transfection with siRNA (time = 0) and then at time = 3, 5, 7, and 9 days. Overexpression of siRNA-resistant myc-SFPQ rescued cell growth in SFPQ knockdown cells. Data are an average n = 3 independent experiments ± s.d. Western blot (below) shows the level SFPQ protein over time. c Knockdown of SFPQ (siSFPQ9) leads to increased apoptosis, quantified by staining for Annexin-V
