Fig. 2
From: Characterization of a long overlooked copper protein from methane- and ammonia-oxidizing bacteria
Structural comparison of PmoDMet49242_1452 to CuA-containing domains. a Crystal structure of PmoDMet49242_1452 periplasmic domain. The asymmetric unit of the crystal is shown as a ribbon diagram, with chain A in magenta and chain B in pink. The copper ion is depicted as a blue sphere and the coordinating residues as well as the two invariant cysteines are shown in stick format. b Comparison of PmoDMet49242_1452 structure (left; magenta) to the CuA-containing domain of T. thermophilus CcO (PDB: 2CUA). Secondary structure elements are labeled, and the CuA site in CcO is indicated with Cu ions shown as spheres and coordinating ligands in stick format. c Superposition of PmoDMet49242_1452 periplasmic domain (magenta) with CcO (gray) colored as in (b). The box indicates the region containing the PmoDMet49242_1452 candidate metal binding ligands, and the inset shows a close-up of the same, shown in stick format. d Model depicting how PmoDMet49242_1452 can form a CuA-like site as a dimer. The two molecules are shown as pink and magenta cartoons, with the residues identified as ligands indicated
