Fig. 3
From: Characterization of a long overlooked copper protein from methane- and ammonia-oxidizing bacteria
In vivo characterization of PmoDMettrDRAFT_0381. a Growth curves of wild-type (black) and ΔpmoD (pink) Methylosinus trichosporium OB3b under copper starved conditions. Error bars represent s.d., n = 5–6. b Growth curves of wild-type (black) and ΔpmoD (pink) Methylosinus trichosporium OB3b grown in the presence of 10 μM CuSO4. Error bars represent s.d., n = 5–6. c qPCR analysis of pmoD genes in Methylosinus trichosporium OB3b at four timepoints (0 min at 0 μM CuSO4, 15 min at 12.5 μM CuSO4, 5 h at 12.5 μM CuSO4, 24 h at 12.5 μM CuSO4). Regulatory changes are shown as log10-transformed normalized relative quantities (NRQs). The four-digit code beneath the gene name represents the locus tag (in MettrDRAFT_NNNN format). Two non-pmoD genes are included for comparison: components of the two MMOs, pMMO (pmoA_0383) and sMMO (mmoX_2362), which are reciprocally regulated by copper. The clearest copper-responsive regulation is observed for the copC-adjacent pmoD MettrDRAFT_0039 (the genes are both copper down-regulated, and regulatory changes are correlated with those observed for components of the mmo and mbn operons) and for the pCuAC-adjacent pmoD MettrDRAFT_3934 (the genes are both copper up-regulated, and regulatory changes are correlated with those observed for components of the pmo operon and csp1). Error bars represent standard error between three biological replicates, each containing three technical replicates. Data points are shown in Supplementary Figure 5
