Fig. 2
Microglia-specific gene and protein expression. a Time course of mRNA expression levels of early microglia markers and factors involved in microglia development in organoids assessed by qRT-PCR and normalized to the geomean of the reference genes SDHA2 and ACTB. Data points represent the mean of four batches of two organoids per batch per timepoint. All batches consisted of organoids derived from iPSC 1. Error bars represent the standard error of the mean (SEM). See also Supplementary Fig. 3a. b, c Double immunostainings of IBA-1 combined with nuclear PU.1 (b) and CD68 (c) at day 52. Representative pictures of cerebral organoids from iPSC 1 are shown. Scale bars 40 µm
