Fig. 3

TBX2 is part of a core-regulatory circuitry. a Significant (Fisher test p-value < 2.2e−16) overlap (min. overlap = 20 bp) of the TBX2 (qval < 0.05), H3K27ac (qval < 0.05), H3K4me3 (qval < 0.05) ChIP-seq and ATAC (qval < 10^−6) peaks in the IMR-32 cell line. b Heatmap profiles −4 kb and +4 kb around the summit of TBX2 peaks. The heatmaps represent the ChIP-seq overlap of TBX2, H3K27ac, ATAC, H3K4me3, and MYCN peaks in the IMR-32 cell line, the histone mark H3K4me1 and the CRC genes GATA3, PHOX2B, and HAND2 in the CLB-GA cell line, and GATA3 in the SH-SY5Y, BE2, NGP, and BE2C cell lines, grouped for promoters or enhancers (homer annotation), and ranked according to the sums of the ChIP-seq peak scores across all ChIP-seq peaks in the heatmap. c TBX2 region (59.1 Mb–59.65 Mb) with the TBX2, ATAC, H3K27ac, and H3K4me3 ChIP profiles in IMR-32 and GATA3, HAND2 and PHOX2B ChIP profiles in CLB-GA. At the bottom, the Lilly called SEs are annotated for the two replicates in IMR-32 as well as the cluster of SEs used for the prioritization (Fig. 1c)