Fig. 1 | Nature Communications

Fig. 1

From: Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate

Fig. 1

Pol µ dGMP and 8-oxodGMP insertion coupled with ligation. a Lane 1 is the minus enzyme control for the single-nucleotide gapped DNA substrate with template base T. Lanes 2–4 and 5–7 are the reaction products in the presence of dGTP and 8-oxodGTP, respectively, and correspond to time points of 10, 30, and 60 s. The position of 5′-adenylate product is indicated as magenta arrow. b Graph shows time-dependent changes in the products of insertion (pink and purple for 8-oxodGTP and dGTP insertions, respectively) and ligation (cyan and blue for ligation following insertion of 8-oxodGTP and dGTP, respectively). The data represent the average of four independent experiments ± SD. Corresponding uncropped gel image and presentation of the data in line graph format are shown in Supplementary Fig. 2

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