Fig. 2
From: Epigenetic profiling for the molecular classification of metastatic brain tumors
DNA methylation differences among brain metastases. a Boxplot representing overall Spearman’s ρ correlation coefficients among brain tumors. The left plot includes all the GBM specimens (n = 60, mean ρ = 0.90 ± 0.06) and all the BM specimens (n = 94, mean ρ = 0.77 ± 0.02). The right plot describes the overall Spearman’s ρ correlation coefficient among BM specimens with anatomical pathology confirmed tumor of origin (BCBM n = 28, LCBM n = 18, and MBM n = 44). The top and bottom of each box represent the first and third quartile, respectively; the internal line represents the median. ***Spearman’s correlation test; P-value < 0.001. b Unsupervised hierarchical clustering using Euclidean distance of the top 5000 most variable genomic regions. c PCA using 31,818 CpG sites with significant (ANOVA, Bonferroni adjusted P-value < 0.05; Supplementary Data 4) differential DNA methylation level among BM with anatomical pathology confirmed tissue of origin (n = 90). d PCA using the differentially methylated region including four BM specimens with uncertain primary tumor of origin (n = 94). e PCA including BM specimens from female patients (n = 58)
