Fig. 7
Constitutive recruitment of Cdc14 to SPBs suppresses the cytokinetic defects of GAL1-DMA2 cells. a BUD4 and GAL1-DMA2 BUD4 cells expressing Cdc14-eGFP at endogenous levels were imaged by time lapse fluorescence microscopy at 30 °C every 2 min. Scale bar: 5 μm. b GAL1-DMA2 BUD4 cells expressing Nud1-GBD at endogenous levels and Cdc14-GFP from a centromeric plasmid were imaged at 30 °C every 4 min in selective medium (−His containing raffinose and galactose) after being induced for ~90 min with galactose. TL transmitted light. Arrowheads indicate the appearance of Cdc14-eGFP at the bud-directed SPB in anaphase. Scale bar: 5 μm. c Cells with the indicated genotypes (all BUD4) were grown in selective medium (−His containing raffinose) at 25 °C, arrested in G1 with alpha factor, induced with galactose 30 min before the release and finally released in YEPRG at 30 °C. At the indicated times cells were collected for FACS analysis of DNA contents. FACS data were plotted after gating out the debris as illustrated in Supplementary Fig. 12
