Fig. 1
From: CDC20B is required for deuterosome-mediated centriole production in multiciliated cells
Single-cell RNA-seq analysis reveals MCC transcriptome at deuterosome stage. a Experimental design of the scRNA-seq experiment. b tSNE plot. Each point is a projection of a unique cell on a 2D space generated by the tSNE algorithm. Blue dots represent MKI67-positive proliferating cells, and red dots represent DEUP1-positive cells corresponding to maturing MCCs at deuterosome stage. c Cell cycle-related gene set expression in HAECs measured by scRNA-seq. Cells were ordered along a pseudotime axis, defined with the Monocle2 package. Phase-specific scores are displayed in the top heatmap. Expression of selected genes is displayed in the bottom heatmap. d tSNEs plots for a selection of genes specifically enriched in deuterosome stage cells. Note that CDC20B exhibits the most specific expression among deuterosome marker genes
