Fig. 2
From: CDC20B is required for deuterosome-mediated centriole production in multiciliated cells
Composition and organization of vertebrate deuterosomes a, b Maturing mouse ependymal MCCs were immunostained as indicated, pictures were taken with confocal (a) or STED (b) microscope. a Individual deuterosomes (dashed boxes in top panels) are shown at higher magnification in bottom panels. DEUP1 stains the deuterosome core (ring) and a close fibrous area that defines the perideuterosomal region. The centriolar marker FOP reveals procentrioles arranged in a circle around the deuterosome. Pericentrin (PCNT) is enriched in the perideuterosomal region. γ-Tubulin (γ-TUB) stains the core as well as the periphery of the deuterosome. b STED pictures showing the organization of FOP, PCNT, and γ-TUB around deuterosomes. Individual centrioles identified by FOP staining are pointed out with arrowheads. The diagram was drawn from the adjacent FOP photograph to help reveal the regular concentric organization of nascent centrioles in a typical deuterosomal figure. c Xenopus embryos were immunostained for γ-Tubulin (γ-Tub) and Centrin and high magnification pictures of immature epidermal MCCs were taken. In these cells, Centrin-positive procentrioles grow around γ-Tubulin-positive structures. d Xenopus embryos were injected with Multicilin-hGR and GFP-Deup1 mRNAs, treated with dexamethasone at gastrula st11 to induce Multicilin activity, and immunostained at neurula st18 for γ-Tubulin, GFP, and Centrin. In c and d, zooms (right panels) were made on regions identified by dashed boxes. Scale bars: 5 µm (a, top), 500 nm (a, bottom), 500 nm (b), 10 µm (c, d, large view), 1 µm (c, d, high magnification)
