Fig. 3
From: CDC20B is required for deuterosome-mediated centriole production in multiciliated cells
CDC20B associates to vertebrate deuterosomes. a Double immunofluorescence was performed on mouse tracheal MCCs after 3 days of culture in air–liquid interface. Low magnification confocal panels show coincident CDC20B and DEUP1 staining in several individual MCCs. High magnification on a single MCC reveals the prominent association of CDC20B to large deuterosomes marked by DEUP1 (arrowheads). Note that some smaller deuterosomes do not contain CDC20B (arrows). b Mouse ependymal MCCs were immunostained as indicated, and high magnification confocal pictures of cells with immature and mature deuterosomal figures were taken. In these cells, centrioles revealed by FOP form a ring around deuterosomes. CDC20B staining forms a ring inside the ring of FOP-positive procentrioles indicating that CDC20B is tightly associated to deuterosomes. Note that the CDC20B signal associated to deuterosome increased with their maturation (high magnification pictures of >25 cells per category from two different animals were quantified in the graph; mean values and standard deviations are shown). Unpaired t test vs immature: p = 0.0005 (intermediate, ***); p < 0.0001 (mature, ****). In a and b, zooms were made on regions identified by dashed boxes. c Xenopus embryos were injected with GFP-Deup1 mRNA and immunostained at neurula st18 as indicated. Scale bars: 5 µm (a, b, large view), 1.5 µm (a, high magnification), 500 nm (b, high magnification), 10 µm (c)
