Fig. 4
DNA-PK inhibitors hinders M1 virus-induced IFN antiviral response. a Gene ontology analysis of the pathways that are overrepresented among the differentially expressed genes between the M1-treated or vehicle-treated HCT-116 cells. b Cells were treated with vehicle or IFN-β (10 ng/μl) for 16 h. Induction of IRF-9 was analysed by western blot. c–h HCT-116 cells were treated with vehicle or NU7441 (1 μM, NU represent NU7441) and M1 virus (MOI = 1) for 24 h and then processed for the qRT-PCR to measure IFN-stimulated gene transcripts. n = 3. Data represent the mean ± SD. *P < 0.05; **P < 0.01 by one-way ANOVA. i HCT-116 cells were pre-treated with NU7441 (1 μM) for 1 h and then infected with M1-GFP at an MOI of 10 in the presence or absence of recombinant IFNα (1000 units/ml). GFP expression was monitored 48 h after M1 inoculation. Scale bar: 50 μm
