Fig. 6
The combination of DNA-PK inhibitors and M1 virus-induced enhanced DNA damage response. a Cells were treated with vehicle, NU7441 (1 μM), M1 (MOI = 1) or NU7441/M1 for 36 h, and comet assay was used to assess double-strand breaks (DSBs). b Quantification of tail moment from a. Box-and-whisker plots show the median (horizontal line), interquartile range (box) and min to max of the data. 62, 61, 60 and 64 cells of vehicle, NU7441, M1 and NU7441/M1 groups, were analyzed, ***P < 0.001 by Kruskal–Wallis test. c, d Immunoblots of proteins after infection with M1 virus (MOI = 1) for 0, 24, 36 and 48 h. e, f Cells were exposed to NU7441 (1 μM), M1 (MOI = 1) or a combination as indicated. p-H2AX (a marker of DNA damage response), cleaved-caspase-3 (a marker of apoptosis) and p-DNA-PKcs (target of NU7441) were determined by western blot. g, h Cells were transfected with siRNA targeting DNA-PKcs (48 h), and this was followed by M1 virus infection (MOI = 1) for 48 h. Proteins were examined by western blot
