Fig. 1

DMF inhibits RSK2. a DMF with Michael acceptor reactive carbons marked with red asterisks. b Schematic representation of RSK2^CTKD and position of cysteine residues. c Inhibition of RSK2^CTKD by DMF (squares) and MMF (triangles). Error bars indicate the standard deviation of mean values (n = 3, all data points included). d Covalent modification of RSK2^CTKD by DMF. Purified murine RSK2 (5 nmol) was incubated with ¹⁴C-labelled DMF (25 nmol). DMF binding is reduced by preincubation of RSK2 with 10-fold molar excess of iodoacetamide or GSH, respectively. The + and – signs represent inclusion or absence of the respective components