Fig. 4
From: Dimethyl fumarate is an allosteric covalent inhibitor of the p90 ribosomal S6 kinases
Mutational studies of DMF inhibition in cells. Mutational analysis of HEK293 cells transfected with RSK2, MSK1, or cysteine mutants hereof. HEK293 cells were supplemented with DMF (140 μM) and stimulated with epidermal growth factor (EGF, 1 ng ml–1). a, b Representative western blots from RSK2 and MSK1 mutational analysis respectively. c, d RSK2CTKD and MSK1CTKD activity determined as the autophosphorylation of S386 and S376, respectively, relative to WT. The activity of EGF-induced wild-type was set to 100% (n = 5 and n = 4, respectively; all data points indicated by black, filled circles)
