Fig. 5
From: Defective transcription elongation in a subset of cancers confers immunotherapy resistance
TEdeff impairs the expression of and signaling through the inflammatory response pathways and antigen presentation. a A diagram of pro-inflammatory response pathways relevant to anti-tumor immune surveillance. Blue nodes indicate those that are found among Class I genes. Genes that have been identified as modulators of tumor response to anti-tumor immune attack in Patel et al.35 and Manguso et al.34 are indicated with bold labels. b Ratios of cytoplasmic to nuclear (left), non-polyadenylated to polyadenylated (middle) and uncapped to capped mRNA levels of the key inflammatory response pathway genes in the indicated cell lines (qPCR). c Protein expression of the genes in b in the indicated cell lines. d TEdeff and TEprof cells were stimulated with IFN-α, IFN-γ, and TNF-α for 30 min and the readout measured by immunoblotting. e Immunoblots of the expression of MHC-I and B2M proteins in the indicated cell lines at the baseline and upon stimulation with IFN-γ. f Indicated cell lines were stimulated with FasL for 24 h and the viability was measured. Error bars: standard deviation based on 3 technical replicates
