Fig. 6 | Nature Communications

Fig. 6

From: Heterozygous deletion of chromosome 17p renders prostate cancer vulnerable to inhibition of RNA polymerase II

Fig. 6

RBX1 promotes the POLR2A-dependent RNA transcription. a Global RNA synthesis in the parental and isogenic 17ploss DU145 cells was evaluated by measurement of 5-EU incorporation after Dox-inducible RBX1 knockdown and the intensity was quantified with the CellProfiler Software. Scale bar, 25 µm. b Effect of RBX1 knockdown on the levels of lysine-63 (K63) ubiquitination of POLR2A in the parental and isogenic 17ploss DU145 cells. c qPCR analysis of the levels of short-lived mRNA (FOS and E2F3) compared to the control 5S rRNA in the isogenic DU145 cells with or without Dox-inducible RBX1 knockdown. d qPCR analysis of the in vitro transcription activity using HelaScribe DNA template. Data are representative of three independent experiments and analyze by unpaired two-tailed t-test. Error bars denote SD. ns not significant; **, p < 0.01; ***, p < 0.001

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