Fig. 2
miR-27a controls susceptibility to Mtb. a–c WT or miR-27a-/- mice were infected with Mtb H37Rv for 28 days, and the lungs were subjected to (a) H&E staining. Scale bar, 100 μm; (b) acid-fast staining of bacteria. Scale bar, 20 μm. (c) CFU assay. d–f Mice were infected with Mtb for 28 days, and treated with NC antagomir or miR-27a antagomir alternate days, and the lungs were subjected to (d) H&E staining, Scale bar, 100 μm; (e) Acid-fast staining of bacteria. Scale bar, 20 μm. (f) CFU assay. *p < 0.05 and **p < 0.01 by the Mann-Whitney U test (c, f). Data are representatives of three independent experiments with biological duplicates in each (c, f; mean ± s.e.m. of n = 6 duplicates) or representative of three independent experiments (a, b, d, e)
