Fig. 2
From: Mutual inhibition between PTEN and PIP3 generates bistability for polarity in motile cells
Bistability of PIP3 enrichment tightly coupled to reciprocal PTEN membrane localization. a Confocal images of the PTEN-Halo variants labeled with TMR and PHPKB-eGFP in living cells during serial dilution of LY294002. Scale bar, 5 μm. b Quantification of a. Fluorescence intensities of PTEN-Halo-TMR and PHPKB-eGFP in a small ROI on the cell membrane were measured along the cell periphery in 5 cells at the indicated LY294002 concentrations. The intensities are normalized to mean fluorescence intensity in the cytoplasm. Higher frequencies are shown in hotter colors. The oblique and horizontal lines show the border between two distributions at the highest and lowest inhibitor concentrations in the DdPTEN and HsPTEN-expressing cells, respectively. c Heat maps showing the frequency of PHPKB-eGFP intensity at the indicated LY294002 concentrations obtained from b. d Scatter plots showing fluorescence intensities of GFP-Nodulin and DdPTEN-Halo-TMR (upper) and GFP-Nodulin and PHPKB-RFP (lower) in a small ROI along the cell periphery in the presence (left; n = 25 and 27 cells for DdPTEN and PHPKB-RFP) or absence (right; n = 28 and 23 cells) of 40 μM LY294002. Higher frequencies are shown in hotter colors. e Histograms of fluorescence intensities of DdPTEN-Halo-TMR (upper) and PHPKB-RFP (lower) under conditions where the GFP-Nodulin fluorescence intensity is within the range of 2.0 to 2.9 in the presence (gray) or absence (green) of 40 μM LY294002
