Fig. 7
PINT87aa decides the localization of PAF1 complex on target genes promoter. a 2nd ChIP assay was used to determine the co-occupation of PAF1 complex/PINT87aa in CPEB1's promoter region by using RT-PCR or q-PCR. At least three independent experiments were performed. b ChIP assay was performed in circPINTexon2 stably overexpressed 456 and 4121 cells by using indicated antibodies. PCR products of indicated genes’ promoter were analyzed by using RT-PCR or q-PCR. c PINT87aa was overexpressed or knocked-down in 293T or hNSC cells by using indicated plasmid or shRNA, respectively. ChIP assay was performed by using indicated antibodies and PCR products of CPEB1 promoter region were analyzed by using RT-PCR or q-PCR. b, c At least three independent experiments were performed
