Fig. 1
From: Diffusion-limited association of disordered protein by non-native electrostatic interactions
Binding experiment of immobilized proteins. a Alexa 488 (D, donor) and Alexa 647 (A, acceptor) are attached to cysteine at the C-terminus and 4-acetylphenylalanine at the N-terminus of TAD, respectively. Dye-labeled molecules were immobilized on a polyethylene glycol (PEG)-coated glass surface via a biotin–NeutrAvidin linkage and incubated with unlabeled NCBD in solution. b Donor-labeled barnase was immobilized on a glass surface and incubated with acceptor-labeled barstar
