Fig. 3

SSCs cultured long term with TECs restore spermatogenesis and fertility in mice. a Images of testes from busulfan-treated mice 12 weeks after transplantation with GFP⁺ SSCs co-cultured long term with either TECs or STO feeder cells. Quantification of GFP⁺ SSC colonies is shown on right. Data shown are out of three independent experiments. a’: Bar = 2 mm, b’: bar = 4 mm, c’: bar = 200 μm. *P < 0.05. b Images of testes and GFP⁺ pups 16 weeks after transplantation of GFP⁺ SSCs co-cultured long term with TECs into infertile W/W^v mice. Bar = 2 mm. c PCR of gfp expression in GFP⁺ pups (1, 4) non-GFP⁺ pups (WT), negative control (N/C), positive controls (GFP, GFP) and a no DNA template control (NTC). d Images of testis sections immunostained with the undifferentiated spermatogonia markers PLZF and Cd49f as well as the spermatid marker PNA. Testes were from mice transplanted with GFP⁺ SSCs after long-term co-culture with TECs. Data shown are out of six independent experiments. Two-tailed unpaired T-test