Fig. 2
Expression of lytic EBV genes in NPC PDXs. a The expression of BZLF1, BRLF1, BMRF1 and BLLF1 transcripts was determined by RNAscope® in situ hybridization. Positive signals are shown as brown dots. Clusters of hybridization signals over NPC cells were interpreted as cells undergoing active lytic EBV infection. Abundant expression of lytic EBV genes was found in all newly established PDXs, but not in C15 or human NPC specimens (NPC1 and NPC2). Scale bar, 100 μm. b Expression of EBV lytic genes in four long-term passaged and four newly established NPC PDXs (Xeno23, 32, 47 and 76) quantified by real-time PCR. The newly established PDXs exhibited higher levels of lytic gene expression. Data are shown as mean ± SD from three independent experiments
