Fig. 1
Sedimentation velocity analysis of NISTmAb at 10 mg/ml. Interference data of NISTmAb stock solution in 25 mM histidine buffer, sedimenting at 45,000 rpm in 3 mm centerpieces, are shown with color temperature gradient indicating the boundary migrating from left to right (circles, for clarity only every 10th point is shown). The sedimentation model accounting for nonideality, cNI(s0), is shown as solid lines, leading to an rmsd of 0.015 fringes. For comparison, the best-fit standard ideal model c(s) is shown as dashed lines, leading to an rmsd of 0.098 fringes. In the nonideal model the frictional ratio is fixed to 1.6, whereas in the standard ideal model the frictional ratio is refined to a best-fit value of 3.5. The lower panel shows residuals of the cNI(s0) fit
