Fig. 8
From: Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion
Cellular defects by knock-in mutation (ILK L207W). a Comparison of focal adhesions and stress fibers between WT ILK containing HeLa cells and ILK L207W knock-in cells. b Western blot of whole-cell lysates from CRISPR generated clones showing that ILK L207W had little effect on the expression of ILK, PINCH, and Parvin. c, d Quantification of focal adhesion number (c) and size (d) by WT ILK vs ILK L207W. e Cell spreading defects by ILK 207W. The box of the boxplot illustrated the upper and lower quartile for each population (WT ILK and ILK L207W). Median is marked by a horizontal line within the box. The attached whisker indicates the range, and the discrete points (•) are the outliers. f WT ILK but not the L207W mutant in HeLa cells promotes cell migration significantly. Images were captured after 10 hours migration, under 10x magnification (left and middle panels). Quantitative change of cell migration by WT ILK versus ILK L207W mutant (right panel). The migrated cell number was calculated from average of five randomly picked fields for each insert. The results were obtained from three independent experiments. Values are given as mean ± S.E.M .Significance was calculated by t test or Mann–Whitney rank sum test based on the normality of data. Scale bar, 20 µm. ***P < 0.001. **P < 0.01
