Fig. 3
From: Structural insights into cGAMP degradation by Ecto-nucleotide pyrophosphatase phosphodiesterase 1
Mutational analysis. a Hydrolytic activities of wild-type (WT) and the S514L mutant of ENPP1 toward 2′3′-cGAMP or ATP. Data are means ± s.d. (n = 3). b IFN-β suppression by WT and the S514L mutant of ENPP1. The ENPP1 protein was expressed in THP-1 cells, and the IFN-β induction was then examined by a luciferase assay. Data are means ± s.d. (n = 3). One-way ANOVA followed by a post-hoc Tukey HSD test was performed. The cell lysates were immunoblotted with the indicated antibodies. NS, not significant
