Fig. 5
From: Structural insights into cGAMP degradation by Ecto-nucleotide pyrophosphatase phosphodiesterase 1
Proposed mechanism of the ENPP1-catalyzed 2′3′-cGAMP degradation. a ENPP1 accommodates the adenine and guanine bases of 2′3′-cGAMP in the N-pocket and G-pocket, respectively. The Thr238 Oγ atom nucleophilically attacks the phosphorus atom in the 2′-5′ phosphodiester linkage, resulting in the production of the pApG intermediate. b The pApG intermediate is flipped over, and the guanine base of pApG is then accommodated in the N-pocket. c The 3′-5′ phosphodiester linkage of pApG is hydrolyzed to produce 5′-AMP and 5′-GMP
