Fig. 3 | Nature Communications

Fig. 3

From: Onsite GTP fuelling via DYNAMO1 drives division of mitochondria and peroxisomes

Fig. 3

Localization of DYNAMO1 in MD and POD machineries. a Phase contrast and immunofluorescence microscopy images of DYNAMO1, Dnm1, Mda1 (anti-Mda1 antibody), FtsZ1 (anti-FtsZ1-antibody), and PDR1 (marker protein for PD machinery, anti-PDR1-antibody) on an isolated MD/PD machinery complex. b Phase contrast and immunofluorescence microscopy images of DYNAMO1 and Dnm1 on isolated POD machineries with diameters of >500 and <400 nm. c Whole-mount negative-staining immunoelectron microscopy images of DYNAMO1 (10 nm immunogold particles) and Dnm1 (15 nm immunogold particles) on isolated POD machinery. Right panel shows a magnified image of the red-boxed area in the left panel. The white arrowhead indicates a filamentous ring serving as a skeletal structure of POD machinery. The green arrowhead indicates a DB ring generating motive force for constriction. Scale bars: 1 μm (a); 500 nm (b); 200 nm (c); 50 nm (magnified image in c)

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