Fig. 2
From: Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation
Characterization of the long non-coding RNA, SVALKA. a LUCIFERASE (LUC) activity of two different CBF1-LUC constructs. White markers indicate three independent transformants of a construct where LUC expression is driven by the CBF1 promoter and terminated by the TNOS terminator (CBF1:TNOS). Black markers indicate three independent transformants of constructs where LUC expression is driven by the CBF1 promoter with the endogenous CBF1 terminator and full SVALKA sequence (CBF1:SVK). The CBF1:SVK lines showed a decreased LUC activity following cold exposure compared to the CBF1:TNOS) lines. LUC activity was measured as average pixel intensity of at least 5 seedlings for each time point. Markers represent mean with standard deviation. Source data are provided as a Source Data file. b Representative images of lines in control conditions and after 9 h of cold exposure for the two LUC constructs. Scale bar represents 1 cm. c Graphical representation of the insertion positions of the T-DNA lines used in this study. The qPCR probes are indicated with red and used in (d) and (e). See Supplementary Figure 5 for additional information on the insertion mutants. d, e Relative SVK (d) and CBF1 (e) expression determined by RT-qPCR in WT and mutants that effect SVK following exposure to cold. Bars represent mean (black: WT, dark grey: uns-1, light grey: svk-1, white: SVK OE, ±SEM) from three biological replicates (rings). The relative level of SVK and CBF1 transcripts were normalized to the level in WT in control conditions. Statistically significant differences between means were determined with Student’s t-test (*p < 0.05, ***p < 0.001). Source data are provided as a Source Data file
