Fig. 3
From: Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation
Mutants that affect SVK show increased freezing tolerance. a Relative level of different cold-responsive transcripts in control and after 24 h of cold exposure in WT, svk-1 and uns-1 determined by RT-qPCR. Bars represent mean (black: WT, dark grey: uns-1, light grey: svk-1, ±SEM) from three biological replicates (rings). The relative level of the COR transcripts were normalized to the level in WT in control conditions. Statistically significant differences were determined with Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001). Source data are provided as a Source Data file. b Electrolyte leakage in non-acclimated (left panel) and cold-acclimated (right panel) WT (black), svk-1 (light grey) and uns-1 (dark grey) plants. Leaf discs of each genotype were in contact with deionized water in a test tube and exposed to −2 °C for 1 h followed by a decreased temperature at a rate of 2 °C/h. Samples were taken out at the indicated temperatures and the solution in the tubes was measured for electrolytes. Subsequently, each tube was submersed in liquid nitrogen allowed to thaw and measured again for electrolytes. Each marker represents the mean electrolyte leakage (electrolyte content before exposure to liquid nitrogen/total electrolyte content) after freezing test compared to the total electrolyte content (±standard deviation) from at least three biological replicates. Source data are provided as a Source Data file
