Fig. 3
From: A low-gluten diet induces changes in the intestinal microbiome of healthy Danish adults
A low-gluten diet alters the functional potential of the gut microbiome. a Microbial genes annotated to Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologs (KOs) were grouped into KEGG modules and manually curated (customised) modules. The bar chart display the median log2 fold change (median ± SEM) of all individual KOs within a module when comparing the relative abundance at baseline to the abundance following the low-gluten diet (blue bars) or the high-gluten diet (red bars), respectively. b Dot plot of the negative log10 of the adjusted P value from the linear mixed model comparing changes in the abundance of modules induced by the low-gluten diet with the changes induced in the high-gluten diet (black dots) adjusting for age, gender, intestinal transit time, participant (n = 51) and carry-over effect. The same analysis was carried out while removing the significant MGSs from the data (grey dots) to elucidate their contribution to the significance. All effect sizes and SEM for each KO can be found in Supplementary Data 3 and 4. c Prevalence of the module across the 1264 MGSs identified from the IGC catalogue16,17. A module was assessed to be present or partially present in a MGS when at least two KOs from the module were detected in the MGS. d Bar plot showing the fraction of the total abundance of a module contributed by each significantly different MGS in per cent. (Supplementary Data 2)
