Fig. 2
From: Sex specific function of epithelial STAT3 signaling in pathogenesis of K-ras mutant lung cancer
Sex-differential immune expression programs in lungs of epithelial-specific K-ras mutant Stat3 deficient mice. a Whole-transcriptome sequencing of whole lungs from 14-week-old CC-LR and LR/Stat3Δ/Δ male and female mice (n = 3 within each genotype and sex group; n = 12 total) was performed using the Ion Torrent Proton platform and as described in the Methods section. Differentially expressed transcripts (n = 339) were identified using a mixed-effects model as described in the Methods section and analyzed by clustering. Rows represent gene features and columns denote samples (yellow, upregulated compared to median sampled; blue, downregulated expression). b Differentially expressed transcripts were functionally and topologically analyzed by pathways and gene set analysis using IPA as described in the Methods section. The gene–gene interaction networks depict significantly predicted inhibition of leukocyte migration and associated gene sets in Stat3-deleted males with the opposite pattern in the female counterparts (orange, activated molecular function; blue, inhibited molecular function; red, upregulated expression; green, downregulated expression)
