Fig. 7
From: Tissue-resident memory T cells populate the human brain
Enrichment CTLA-4 and PD-1 expression on brain CD8+ T cells. a, b, d, e Quantification of the percentage of CD8+ T cells expressing PD-1 and CTLA-4, respectively. Bars show median values. p-values show Mann–Whitney U test for unpaired data (a, d) and Friedman test for paired data with Wilcoxon signed ranks as post hoc test (b, e) (*p < 0.05, **p < 0.01); no brackets indicate no significant difference. Representative dot plots of CD3+CD8+ lymphocytes stained for c PD-1 and CD103 and f CTLA-4 and CD103. Immunohistochemical staining for g CD86 (ligand of CTLA-4, brown) and h PD-L1 (ligand of PD-1, brown) in a donor with Alzheimer’s disease and a donor without brain disease showed no staining (scale bar = 50 µm). However, in an HLA-DR-positive active, demyelinating MS lesion of a donor with MS, specific staining for i CD86 and j PD-L1 was found in microglia and astrocyte-like cells, respectively (scale bar = 50 µm; scale bar insert = 20 µm)
