Fig. 1
From: Locally anchoring enzymes to tissues via extracellular glycan recognition
Design of G3 fusion proteins to locally anchor enzymes to tissues via extracellular glycan binding. a–c Schematic of recombinant enzymes fused with galectin-3 (i.e., enzyme-G3 fusion protein), which are anchored to an injection site via binding to cell surface and extracellular matrix (ECM) glycans. d Monomeric fusion protein consisting of an enzyme linked to the N-terminal domain of galectin-3 via a flexible peptide linker. e Trimeric nanoassembly formed by inserting the TT domain between the enzyme and G3 domains. The trimeric nanoassembly has higher glycan-binding affinity than the monomeric fusion protein due to multivalent avidity effects. PDB ID: 253L [10.2210/pdb253L/pdb] (generic enzyme), 2O7H [10.2210/pdb2O7H/pdb] (generic coiled-coil), and 5NF7 [10.2210/pdb5NF7/pdb] (carbohydrate-recognition domain of galectin-3)
