Fig. 5
From: Locally anchoring enzymes to tissues via extracellular glycan recognition
Injection site half-life of monomeric G3 fusion proteins and trimeric nanoassemblies. a Bioluminescence images and b photon flux at various time points for mice that received NL-TT-G3, NL-G3, or WT-NL (equivalent moles of NL) in the hock, scruff, or thigh. c Biocatalytic activity half-life of NL-TT-G3, NL-G3, or WT-NL in different tissues. N = 5, mean ± s.d., *p < 0.05, ANOVA with Tukey’s post hoc. In c, open circles represent each half-life calculation, closed circle represents individual animals for which half-life could not be accurately calculated due to rapid enzyme clearance from the injection site, and hash sign represents groups for which half-life could not be accurately calculated due to rapid enzyme clearance from the injection site (signal at t = 24 was at baseline for ≥3 animals in a cohort). Data for WT-NL appear as gray bars/squares/traces, NL-G3 as blue bars/circles/traces, and NL-TT-G3 as red bars/triangles/traces
