Fig. 6
From: Locally anchoring enzymes to tissues via extracellular glycan recognition
Circulating concentration and proteolysis of monomeric G3 fusion proteins and trimeric nanoassemblies. a Percentage of NL, by total mass injected, in blood samples collected over time after subcutaneous injection into the hock. b Percentage of NL activity, relative to NL activity at the initial time point, in 25% mouse serum in vitro. c Schematic of ECM proteases, such as collagenase and other MMPs, digesting the N-terminal domain (NTD) of G3, thereby separating the carbohydrate-recognition domain (CRD) of G3 from the enzyme fusion partner. d SDS-PAGE analysis of collagenase-mediated digestion of WT-G3, NL-G3, and NL-TT-G3. Uncropped gel image found in Supplementary Figure 26. N = 5, mean ± s.d. for a. N = 3, mean ± s.d. for b. Data for NL-G3 appear as blue circles/traces and NL-TT-G3 as red triangles/traces
