Fig. 5
Loss of actomyosin regulators results in reduced cytoplasmic streaming. a Representative images of the average velocity vectors (PIV analysis) of the cytoplasmic streaming in the distal region of control, cyk-1(or596) at 20 °C, nmy-2(RNAi) on L4 stage worms, cyk-1(RNAi) on cyk-1(or596), nmy-2(RNAi) on L3 stage worms, and mel-11(RNAi) gonads. Images are oriented in the direction of distal (D) to proximal axis (P) of the gonad. b, c Average speed and average velocity of the cytoplasmic streaming (along the X-axis) in the distal region of control (N = 17), cyk-1(or596) at 20 °C (N = 22), nmy-2(RNAi) on L4 stage worms (N = 10), cyk-1(RNAi) on cyk-1(or596) (N = 11), nmy-2(RNAi) on L3 stage worms (N = 10), and mel-11(RNAi) gonads (N = 19). The particle speed and velocity was analyzed for 200 s in each movie. Data represents mean ± s.d. of four independent experiments. Statistical analysis was done using one-way ANOVA test (*p < 0.05, **p < 0.005, ***p < 0.0001, ns, non-significant), N = number of gonads analyzed. Scale bar, 5 µm
